Capillary Electrophoresis-Mass Spectrometry by James Q. Xia download in ePub, pdf, iPad
As the ball rotates the sample is dried before it reaches ionization region. This technique has high sensitivity since no makeup fluid is used. There are number of studies reporting characterization of mixtures of peptides and proteins. However, the sensitivity is reduced and the mixing of two liquids could degrade separation.
The separation and detection of analytes can be improved with better interface. This book outlines the potential of this technique for researchers working in metabolomics, bioanalytics and biomarker analysis. This interface system had few drawbacks like mismatch in the flow rates of two systems. The separated ions are then measured using a detector. Along with that, it is used often for routine analysis of pharmaceutical drugs.
Since then, interface system has been improved to have continuous flow rate and good electrical contact. Intrigued by metabolomics for disease prediction and diagnosis, Rawi switched to the Leiden University Medical Center to broaden his horizon on this topic. Mass spectrometry is an analytical technique that identifies chemical species depending on their mass-to-charge ratio.
Analytes migrate from one end of capillary to other based on their charge, viscosity and size. Higher the electric field, greater is the mobility. The electrical contact is established by makeup liquid surrounding the junction between two capillaries. This system is easy to operate.
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